Cas9HF1 enhanced specificity in Ustilago maydis
نویسندگان
چکیده
منابع مشابه
Specificity of Ustilago maydis killer proteins.
Bacteria and fungi were tested for sensitivity to Ustilago maydis killer strains carrying virus-like particles. Various species taxonomically related to U. maydis were sensitive.
متن کاملسیتولوژی Ustilago maydis
رفتار هسته در تلیوسپور، بازیدیوسپور و میسلیومهای Ustilago maydis مورد بررسی قرار گرفت. جهت کشت تلیوسپورها از محیط PDA + 10% دکستروز استفاده گردید. مطالعه رفتار هسته با استفاده از میکروسکوپ اپیفلورسنت و رنگآمیزی با دو رنگ اتیدیوم برماید و اکردین اورنج صورت پذیرفت. هسته در تلیوسپور کروی و بزرگ و اکثر فضای داخلی آن را اشغال نموده است. بازیدیوسپورها (اسپوریدیوم) به صورت غیرانگلی و به روش جوانهز...
متن کاملGlycolysis in Ustilago maydis.
The kinetic parameters of the 10 glycolytic enzymes and glycolytic fluxes were determined for the first time in Ustilago maydis. Enzyme activities in yeast grown in minimal medium and harvested in the stationary stage were twofold higher than those from yeast grown in rich medium. In contrast, in yeast harvested in the exponential stage, the enzyme activities were higher in cells grown in rich ...
متن کاملUstilago maydis as a Pathogen.
The Ustilago maydis-maize pathosystem has emerged as the current model for plant pathogenic basidiomycetes and as one of the few models for a true biotrophic interaction that persists throughout fungal development inside the host plant. This is based on the highly advanced genetic system for both the pathogen and its host, the ability to propagate U. maydis in axenic culture, and its unique cap...
متن کاملInitiation of meiotic recombination in Ustilago maydis.
A central feature of meiosis is the pairing and recombination of homologous chromosomes. Ustilago maydis, a biotrophic fungus that parasitizes maize, has long been utilized as an experimental system for studying recombination, but it has not been clear when in the life cycle meiotic recombination initiates. U. maydis forms dormant diploid teliospores as the end product of the infection process....
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ژورنال
عنوان ژورنال: Fungal Biology
سال: 2020
ISSN: 1878-6146
DOI: 10.1016/j.funbio.2020.02.006